Tuesday, January 21, 2014

Electron Microscope

electron Microscope An electron microscope is a microscope that uses a beam of electrons sort of than light. negatron microscopes have a much better sequent than standard ocular microscopes . This is because the wavelength of visible light is very massive and so generates an go for of poor resolution. negatron microscopes however realize much higher levels of resolution because of the very short wavelength of the electron bean which is used. The electron beam is negatively charged and so electromagnets can be used to focus the image this allows a greater level of precision than would be available in a normal opthalmic microscope when trying to focus the image. negatron microscopes can resolve objects that be 0.1 nm apart which is 2000 total more than than you can resolve with a standard visual microscope. Because the electrons which ar used in the EM microscope are deflected by particles in the air the microscope has to be kept at a near vacuum state in order for a useful image to be seen. EM microscopes also have the object that is being magnified to be dead whereas optical microscopes can view live cells and hence show constitute of what is happening rather than just a freeze frame. negatron microscopes also cost a significant amount of currency to buy and maintain.
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It is also possible for electron microscopes to have artefacts which are structural details caused by the preparation of the exemplification. TEM Transmission electron Microscope A beam of electrons is passed through a thin object. more or less areas absorb the electrons and others dont allowing an image to be created. fuelt be done on livi! ng specimens. A complex staining process is necessary even if no colour is penuryed in the final image. The specimen must(prenominal) be extremely thin for the electrons to pass through it. It gives a flat 2 dimensional image. SEM Scanning Electron Microscope The SEM has the alike(p) limitations as the TEM in that it can only done on dead specimens and it requires a complex staining process however...If you pauperism to get a full essay, order it on our website: OrderCustomPaper.com

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